TRACP 5b Assays (Tartrate-resistant Acid Phosphatase 5b)

Immunodiagnostic Systems
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TRACP 5 & TRACP 5b – What is the difference?

TRACP 5 assays detect Tartrate-Resistant Acid Phosphatase released from macrophages (TRACP 5a) and osteoclasts (TRACP 5b) whereas the BoneTRAP Assay employs a highly specific monoclonal that detects only active enzyme released from viable osteoclasts.

Approximately 90% of circulating TRACP is in the 5a isoform, therefore it is important to use a method which is specific for osteoclast-derived TRACP 5b when assessing bone resorption.

Tartrate-Resistant Acid Phosphatase Assay

BoneTRAP Assay (TRACP 5b)

  • Measures specifically osteoclast-derived TRACP 5b and correlates with osteoclast number.
  • Detects only enzymatically active TRACP 5b molecules freshly released from osteoclasts.14
  • No interference from enzymatically active TRAP 5a molecules or circulating inactive TRACP fragments.14
  • Low diurnal variability, no dietary influences, and no accumulation in renal or hepatic failure.
  • Also available as MouseTRAP™ Assay (mouse) and RatTRAP™ Assay (rat) formats.
  • More than 100 publications documenting scientific principles and applications.
  • Patented TRACP 5b technology.
  • BoneTRAP assays have been used for investigation into:
    • Detecting the spread of tumour cells to bone in cancer research subjects
    • Detecting increased bone resorption rate in subjects with bone diseases such as osteoporosis and renal bone disease
    • Assessing the efficacy of anti-resorptive treatment
    • Quantitating bone resorption and osteoclast number in human, rat and mouse osteoclast cultures
    • Fracture Risk

An emerging biomarker with unique applications in:

Bone Disease
Bone Resorption1,2,3
Osteoporosis2,4
Paget's Disease5

Cancer
Bone Metastases2,6,7,8,9
Multiple Myeloma10,11

Chronic Kidney Disease
Haemodialysis12
Transplant follow-up13

References:

  1. Nenonen A et al. J Bone Miner Res 20:1804-1812, 2005
  2. Halleen JM et al. Clin Chem 47:597-600, 2001.
  3. Hannon RA et al. Bone, 2004 Jan; 34(1):187-194, 2004.
  4. Gerdhem P et al. J Bone Miner Res 19:386-393, 2003.
  5. Durham BH et al. Calcif Tissue Int. 72(4):P11, 2003 (Abstract)
  6. Chao TY et al. J Biomed Sci 11:511-516, 2004.
  7. Jung K et al. Int J Cancer 111:783-791, 2004.
  8. Koizumi M et al, Anticancer Res 23:4095-4099, 2003.
  9. Mose S et al, Anticancer Res 23:2783-2788, 2003.
  10. Koenigsmann M et al. Bone 36:Supp.2-P374, 2005 (Abstract)
  11. Terpos E et al, Int J Cancer 106:455-457, 2003.
  12. Avbersek-Luznik I et al. Nephrol Dial Transplant 20:566-570, 2005.
  13. Malyszko J et al. Tranplant Proc 35:1351-1354, 2003.
  14. Halleen JM et al. J Bone Miner Res 15:1337-1345, 2000.

For more information also download:
TRAP scientific paper
TRAP flyer

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