SubscribeSAINT-MIX is based on a synthetic amphiphilic (non-liposomal) delivery system combined with DOPE (Dioleoyl-phosphatidylethanolamine).
The pyridinium surfactant of SAINT-MIX enables non-toxic delivery of up to 111kb DNA (linear or plasmid), RNA, oligonucleotides. SAINT-MIX / DNA complexes have been successfully used in vitro and in vivo.
SAINT-MIX product description:
The SAINT-MIX delivery system is based on a cationic pyridinium head group, showing excellent bio-compatibility.
Molecular structure of SAINT-18 (1-methyl-4-(cis-9-dioleyl) methyl-pyridinium-chlorid):
SAINT-MIX consists of SAINT and DOPE (Dioleoyl-phosphatidylethanolamine) dissolved in H2O. The SAINT: DOPE ratio is 1:1. SAINT-MIX is supplied in 2 glass vials, each containing 2ml SAINT-MIX in a 0.75mM concentration. Under standard conditions (1µg DNA complexed with 15nmol of SAINT-MIX) a standard package is sufficient to perform transfection experiments with 200 µg DNA.
Stability experiments showed that SAINT-MIX, stored at 4°-7°C, is stable for at least one year from the date of manufacture. No loss of function appeared after repeatedly opening of the vials as long as the vials are recapped tightly after use. The antibiotic properties of SAINT-MIX ensure sterility for prolonged periods. (For more information go to stability in R&D News)
SAINT-MIX Applications:
DNA transfection:
A wide variety of mammalian cells can be transfected with SAINT-MIX, including poor transfectable cell-lines such as primary endothelial cells (HUVEV, HUAEC, HV-5).
SAINT-MIX has been used to transfect several cell-types/lines, some with efficiencies up to 100% (see/ LINK General instructions: Cell lines tested)
Oligonucleotides:
SAINT-MIX has been demonstrated to act as an efficient translocator
of oligo-nucleotides into eukaryotic cells. The compatibility with serum is a major advantage of this delivery system. Oligonucleotide induced cell-damage will be minimal.
Protocols:
SAINT-MIX
SAINT-MIX Sketch
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