SubscribeSAINT-PhD protein delivery reagent is based on a synthetic amphiphilic (non-liposomal) delivery system combined with DOPE (Dioleoyl-phosphatidylethanolamine).
The pyridinium surfactant of SAINT-PhD enables non-toxic delivery of proteins.
SAINT-PhD product description:
SAINT-PhD consists of a proprietary cationic pyridinium amphiphile and a helper lipid. Upon mixture of SAINT-PhD with the protein a particle of approximately 200nm in diameter is formed. In this particle the protein is enwrapped by at least one bilayer of lipids. Furthermore, in the complex formed only non-covalent interactions are present between SAINT-PhD and the protein. The cationic amphiphiles on the surface of the particle have high affinity for the negatively charged cell surface. Upon fusion or entrapment of the particle the protein is released into the cytoplasm of the cell. The proteins delivered by SAINT-PhD are functional and unmodified.
A range of different cell types have been tested with SAINT-PhD. Protein delivery efficiencies of up to 100% of the cells have been reached. The cells that successfully have been tested with SAINT-PhD are listed below:
Adherent cell lines:
B16F10
COS-7
HEK-293
SKOV-3
U373
Adherent primary cells:
HUVEC
Suspension cell lines:
Jurkat
Click here to read more about some results of SAINT-PhD
Protocols:
 | Other Products |