SubscribeThis is one of the synthetic part of SAINT-MIX in pure form, without the DOPE part.
SAINT-18 is an alternative delivery system for hard to transfect cell-lines and primary cells. SAINT-18, which is 100% Synthetic, is applied for in vivo experiments when SAINT-Gene-Therapy-Grade is not yet essential.
SAINT-18 product description:
The SAINT-18 delivery system is based on a cationic pyridinium head group, showing excellent bio-compatibility.
Molecular structure of SAINT-18 (1-methyl-4-(cis-9-dioleyl)methyl-pyridinium-chlorid):
SAINT-18 is dissolved in H2O. The SAINT-18 is supplied in a glass vial containing 2ml SAINT-18 in a 0.75mM concentration. Under standard conditions (1µg DNA complexed with 15nmol of SAINT-18) a standard package is sufficient to perform transfection experiments with 100µg DNA.
Stability experiments showed that SAINT-18, stored at 4°-7°C, is stable for at least one year from the date of manufacture. No loss of function appeared after repeatedly opening of the vials as long as the vials are recapped tightly after use. The antibiotic properties of SAINT-18 ensure sterility for prolonged periods.
SAINT-18 Applications:
A wide variety of mammalian cells can be transfected with SAINT-18, including poor transfectable cell-lines such as primary endothelial cells (HUVEV, HUAEC, H5V). Efficiencies of 15% up to 50% have been achieved with minimal cellular damage.
SAINT-18 is intended for pre-clinical in vivo experiments.
Only SAINT-18GT (Gene Therapy Grade) is suitable for clinical experiments. SAINT-18 is not produced under GMP protocols and is therefore not yet allowed to be used in clinical (human) experiments!
Protocols:
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