Automated Vitamin D Clinical Analysis Kit

Now available from Anachem, the new Vitamin D analysis kit from Recipe provides all the reagents necessary for measurement of 25-OH-vitamin D2/3 in patient samples. With Anachem’s liquid handling platform, Flexus, the whole kit protocol can be automated providing fast and efficient processing for more accurate clinical results.

Patient Sample Tracking

Patient samples are tracked through the process with the Flexus bar code readers. The Flexus transfers patient samples from their individual tubes and injects them into to a deep well microtitre plate that contains a precipitant and internal standard from the Recipe kit. The components are mixed together on the Flexus integral plate shaker and the plate is then transferred robotically to the onboard centrifuge. A second plate is prepared with a second set of samples or as a blank to balance the centrifuge. The plates are spun for 10 minutes, before Flexus removes the supernatants to a clean plate, ready for analysis by HPLC. The Flexus automated liquid handler allows you to prepare 192 samples for analysis quickly and reproducibly, giving you time to do other less repetitive and laborious tasks.

Vitamin D Analysis 

Vitamin D analysis using the Recipe kit is well suited to the Flexus as automating this process saves time, reduces error and provides more controlled clinical results faster.

For further information on this procedure please contact Anachem for a copy of the Vitamin D Application Note.

Anachem has been the leading specialist distributor to the U.K. & Irish scientific communities for over thirty five years. Today, Anachem represents leading suppliers, alongside its own brands, to provide world renowned chromatography and laboratory automation solutions.

For further information or to receive a copy of the application note please contact: Anachem Ltd, Charles Street, Luton, LU2 0EB, Tel: 01582 747501.

Editor's Note

Vitamin D is a group of fat soluble, prohormones, similar in structure to steroids, that are essential for the incorporation of calcium into bones. The lack of Vitamin D can cause bone deficiencies including rickets in children and osteomalacia in adults. The normal range of Vitamin D can change between summer and winter due to the amount of exposure to light, which is critical to the synthesis of Vitamin D3 in our skin. Vitamin D2 is absorbed from the food that we eat and from nutritional supplements.